Ustilago maydis secreted T2 ribonucleases, Nuc1 and Nuc2 scavenge extracellular RNA.
Identifieur interne : 000008 ( Main/Exploration ); précédent : 000007; suivant : 000009Ustilago maydis secreted T2 ribonucleases, Nuc1 and Nuc2 scavenge extracellular RNA.
Auteurs : Dibya Mukherjee [Inde] ; Sayandeep Gupta [Inde] ; Abhrajyoti Ghosh [Inde] ; Anupama Ghosh [Inde]Source :
- Cellular microbiology [ 1462-5822 ] ; 2020.
Abstract
Ustilago maydis genome codes for many secreted ribonucleases. The contribution of two among these belonging to the T2 family (Nuc1 and Nuc2) in the pathogen virulence, has been assessed in this study. The nuc1 and nuc2 deletion mutants showed not only reduced pathogenicity compared to the SG200 WT strain but also exhibited significant delay in the completion of the pathogenic lifecycle. Both the proteins were also tested for their nucleolytic activities towards RNA substrates from maize and yeast. This also yielded valuable insights into the ability of the ribonucleases to utilise extracellular RNA as a nutrient source. Our study therefore established a role of two T2 type secreted ribonucleases of a phytopathogen in the acquisition of nutrient for the first time. This study also provides evidence that maize apoplast contains RNA, which can be utilised as a substrate by both Nuc1 and Nuc2.
DOI: 10.1111/cmi.13256
PubMed: 32844528
Affiliations:
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wicri:level="1"><nlm:affiliation>Department of Biochemistry, Bose Institute Centenary Campus, Kolkata, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Bose Institute Centenary Campus, Kolkata</wicri:regionArea><wicri:noRegion>Kolkata</wicri:noRegion></affiliation></author><author><name sortKey="Ghosh, Anupama" sort="Ghosh, Anupama" uniqKey="Ghosh A" first="Anupama" last="Ghosh">Anupama Ghosh</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biology, Bose Institute Centenary Campus, Kolkata, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute Centenary Campus, Kolkata</wicri:regionArea><wicri:noRegion>Kolkata</wicri:noRegion></affiliation></author></analytic><series><title level="j">Cellular microbiology</title><idno type="eISSN">1462-5822</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ustilago maydis genome codes for many secreted ribonucleases. The contribution of two among these belonging to the T2 family (Nuc1 and Nuc2) in the pathogen virulence, has been assessed in this study. The nuc1 and nuc2 deletion mutants showed not only reduced pathogenicity compared to the SG200 WT strain but also exhibited significant delay in the completion of the pathogenic lifecycle. Both the proteins were also tested for their nucleolytic activities towards RNA substrates from maize and yeast. This also yielded valuable insights into the ability of the ribonucleases to utilise extracellular RNA as a nutrient source. Our study therefore established a role of two T2 type secreted ribonucleases of a phytopathogen in the acquisition of nutrient for the first time. This study also provides evidence that maize apoplast contains RNA, which can be utilised as a substrate by both Nuc1 and Nuc2.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">32844528</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1462-5822</ISSN><JournalIssue CitedMedium="Internet"><Volume>22</Volume><Issue>12</Issue><PubDate><Year>2020</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Cellular microbiology</Title><ISOAbbreviation>Cell Microbiol</ISOAbbreviation></Journal><ArticleTitle>Ustilago maydis secreted T2 ribonucleases, Nuc1 and Nuc2 scavenge extracellular RNA.</ArticleTitle><Pagination><MedlinePgn>e13256</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/cmi.13256</ELocationID><Abstract><AbstractText>Ustilago maydis genome codes for many secreted ribonucleases. The contribution of two among these belonging to the T2 family (Nuc1 and Nuc2) in the pathogen virulence, has been assessed in this study. The nuc1 and nuc2 deletion mutants showed not only reduced pathogenicity compared to the SG200 WT strain but also exhibited significant delay in the completion of the pathogenic lifecycle. Both the proteins were also tested for their nucleolytic activities towards RNA substrates from maize and yeast. This also yielded valuable insights into the ability of the ribonucleases to utilise extracellular RNA as a nutrient source. Our study therefore established a role of two T2 type secreted ribonucleases of a phytopathogen in the acquisition of nutrient for the first time. This study also provides evidence that maize apoplast contains RNA, which can be utilised as a substrate by both Nuc1 and Nuc2.</AbstractText><CopyrightInformation>© 2020 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mukherjee</LastName><ForeName>Dibya</ForeName><Initials>D</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-0910-1161</Identifier><AffiliationInfo><Affiliation>Division of Plant Biology, Bose Institute Centenary Campus, Kolkata, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gupta</LastName><ForeName>Sayandeep</ForeName><Initials>S</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-8079-7712</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, Bose Institute Centenary Campus, Kolkata, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ghosh</LastName><ForeName>Abhrajyoti</ForeName><Initials>A</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-2469-3740</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, Bose Institute Centenary Campus, Kolkata, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ghosh</LastName><ForeName>Anupama</ForeName><Initials>A</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-4200-5091</Identifier><AffiliationInfo><Affiliation>Division of Plant Biology, Bose Institute Centenary Campus, Kolkata, India.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>IFA13-LSPA16 (DST-INSPIRE Faculty Scheme)</GrantID><Agency>Department of Science and Technology, Ministry of Science and Technology, India</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Cell Microbiol</MedlineTA><NlmUniqueID>100883691</NlmUniqueID><ISSNLinking>1462-5814</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">T2 ribonucleases</Keyword><Keyword MajorTopicYN="N">Ustilago maydis</Keyword><Keyword MajorTopicYN="N">Zea mays</Keyword><Keyword MajorTopicYN="N">effector proteins</Keyword><Keyword MajorTopicYN="N">extracellular RNA</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>05</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32844528</ArticleId><ArticleId IdType="doi">10.1111/cmi.13256</ArticleId></ArticleIdList><ReferenceList><Title>REFERENCES</Title><Reference><Citation>Abel, S., Nurnberger, T., Ahnert, V., Krauss, G. 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PLoS Pathogens, 15(3), e1007620. https://doi.org/10.1371/journal.ppat.1007620</Citation></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Mukherjee, Dibya" sort="Mukherjee, Dibya" uniqKey="Mukherjee D" first="Dibya" last="Mukherjee">Dibya Mukherjee</name></noRegion><name sortKey="Ghosh, Abhrajyoti" sort="Ghosh, Abhrajyoti" uniqKey="Ghosh A" first="Abhrajyoti" last="Ghosh">Abhrajyoti Ghosh</name><name sortKey="Ghosh, Anupama" sort="Ghosh, Anupama" uniqKey="Ghosh A" first="Anupama" last="Ghosh">Anupama Ghosh</name><name sortKey="Gupta, Sayandeep" sort="Gupta, Sayandeep" uniqKey="Gupta S" first="Sayandeep" last="Gupta">Sayandeep Gupta</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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